Cholera toxin B subunits assemble into pentamers--proposition of a fly-casting mechanism.
The cholera toxin B pentamer (CtxB(5)), which belongs to the AB(5) toxin family, is used as a model study for protein assembly.The effect of the pH on the reassembly of the toxin was investigated using immunochemical, EftPos Cleaning Cards electrophoretic and spectroscopic methods.Three pH-dependent steps were identified during the toxin reassembly